Thioglycolate-elicited
Peritoneal macrophages were harvested using the protocol as described previously [1]. Elicited macrophages were harvested from mice which had received a 1.5 ml intraperitoneal administration of 3% Brewer thioglycollate broth (Sigma, UK) 72 h prior collection. Resident macrophages were also harvested from … See more 12-week old C57BL/6 J mice were bred and maintained in the Biological Service Unit at Queens University Belfast in a 12 h-light/12 h-dark cycle with free access to water and chows. The use of animals was in compliance with … See more AlamarBlue® Cell Viability (ThermoFisher Scientific) was performed according to manufacturer’s guidelines. See more Cell suspensions were processed for staining of cell surface markers, including F4/80 (clone BM8), LFA-1 (clone 2D7), MHCII (clone AF6 … See more Phagocytic activity of macrophages was assessed using the pHrodo S.aureus bioparticles conjugate phagocytosis kit and the Dextran-FITC phagocytosis system (ThermoFisher Scientific, UK) according to the … See more WebSep 20, 2015 · Similarly, thioglycollate-elicited peritoneal cells isolated from wildtype mice treated with TP-064 showed lowered mRNA expression levels and cytokine production of pro-inflammatory mediators ...
Thioglycolate-elicited
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WebJan 1, 2013 · Thioglycollate-challenged AdBRCA1-treated mice displayed reduced peritoneal neutrophil recruitment and dampened cytokine elaboration relative to their Adnull-treated counterparts. Taken together, we report a novel role of BRCA1 gene therapy in limiting systemic inflammation, multiple-organ failure and mortality in experimental sepsis. WebSep 16, 2024 · Enhanced Ca 2+ handling in thioglycolate-elicited peritoneal macrophages Feng Liu, Luxin Xu, Miyuki Nishi, Atsuhiko Ichimura, Hiroshi Takeshima Cell Calcium 96 102381-102381 2024年6月 Total Synthesis of Zephycarinatines via Photocatalytic Reductive Radical ipso-Cyclization Haruka Takeuchi, Shinsuke Inuki, Kohei Nakagawa, Takaaki …
Web4.3.10 Dyeing silk with sulphur dyes. Silk was reported to be dyed with four C.I. Solublized Sulphur dyes using sodium thioglycolate (HSCH2 CO 2 Na), a mild reducing agent at pH 7, … WebStudies of macrophage biology have been significantly advanced by the availability of cell lines such as RAW264.7 cells. However, it is unclear how these cell lines differ from primary macrophages such as thioglycolate-elicited peritoneal macrophages (TGEMs). We used the inflammatory stimulus Kdo2-lipid A (KLA) to stimulate RAW264.7 and TGEM cells. …
WebC57BL/6 Mouse Peritoneal Macrophages (Thioglycollate-elicited) are isolated from the peritoneal cavity of pathogen-free laboratory adult mice. Cells at passage 0 are cryo … WebDec 20, 2011 · Thioglycolate-elicited peritoneal macrophages prepared after four weeks of feeding, and then challenged with LPS (10 ng or 100 ng) resulted in increases of 55% and 73%, respectively in the production of NO of 46-week-old compared to 8-week-old mice fed control diet alone (respective control groups), without affecting the secretion of TNF-α …
WebJun 1, 2024 · Brewer thioglycolate broth is a bacterial culture medium and has been used as a non-selective macrophage stimulant mainly for examining changes in resistance to …
WebApr 9, 2024 · Thioglycolate-elicited macrophages were obtained from the peritoneal cavity of male C57BL6/6J mice as previously described . Briefly, mice were intraperitoneally injected with 3 mL of 3% thioglycolate (BD Biosciences Japan, Tokyo, Japan). Four days later, the exudate cells in the peritoneal cavity were collected by washing with PBS. shop exchange fort hoodWebBALB/c Mouse Peritoneal Macrophages (Thioglycollate-elicited) are isolated from the peritoneal cavity of pathogen-free laboratory adult mice. Cells at passage 0 are cryo-preserved in vials. Each vial contain 6x10 6 cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma and tested for expression of markers ... shop exchange computer i9WebThioglycolate-elicited and bone marrow-derived are the most commonly used primary macrophages. Elicited peritoneal macrophages can be harvested 3 days after … shop exchange customer serviceWebFeb 10, 2024 · The cells can be collected under non-elicited (resident) or elicited (e.g., with Brewer thioglycollate broth injection) conditions, and their phenotype and functions differ. … shop excellenceWebDec 26, 2015 · Thioglycollate elicited peritoneal macrophages can be harvested and cultured in the same way as described in steps 1-15 with additional steps as shown … shop exchange car repairWebThioglycolate-elicited Balb/c peritoneal macrophages primed with IFN-γ (100 ng/ml) for 2 hours LPS (1 μg/ml) 4-6 hours monensin or brefeldin A CD107b (MAC-3) GM-CSF MP1-22E9 Splenocytes stimulated with immobilized anti-CD3 (10 μg/ml) + … shop exchange small businessWebJan 24, 2024 · Primary murine macrophages can be obtained from: bone marrow by different treatments (granulocyte-macrophage colony-stimulating factor—GM-CSF, macrophage colony-stimulating factor—M-CSF or supernatant of murine fibroblast L929), peritoneal cavity (resident or thioglycolate elicit macrophages), from the lung (alveolar … shop executive director