Phocl variant
WebbWe demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening … Webbgenetics, PhoCl has some unique bene cial properties, such as robust heterologous expression, self-sufficient chromophore formation,23 and a uniquely irreversible …
Phocl variant
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Webb9 dec. 2024 · Here, we report the X-ray crystal structures of the PhoCl1 green state, red state, and cleaved empty barrel. Molecular dynamics (MD) simulations were performed to provide insight into the precise dissociation mechanism. Using structure-guided engineering and directed evolution, we have developed PhoCl2c with higher contrast … WebbThe finalized PhoCl system has a broad absorbance from 400 to 500 nm, and the dissociation rate is 500 s. Many proteins, ≈ such as Cre recombinase, Gal4 transcription factor, Pannexin-1 ion channel, could be controlled by PhoCl.[10]
Webb10 dec. 2024 · The photocleavable protein (PhoCl) is a green-to-red photoconvertible fluorescent protein that, when illuminated with violet light, undergoes main chain … WebbWe demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening …
WebbPhoCl optoproteins have also been engineered to tune the number of solventexposed cysteines to create both softening and stiffening OptoGels from identical illumination … WebbThe first generation PhoCl (PhoCl1) exhibited a relative slow rate of dissociation, potentially limiting its utilities for optogenetic control of cell physiology. In this work, we report the X-ray crystal structures of the PhoCl1 green state, red state, and cleaved empty barrel. Using structure-guided engineering and directed evolution, we have ...
Webb13 mars 2024 · An engineered photoconvertible fluorescent protein (pcFP) variant, designated as mMaple, that is suited for use in multiple conventional and super …
Webb25 maj 2024 · The integrin-binding motif RGD was fused to the PhoCl and was decorated on the hydrogel. After being exposed to light at 405 nm, the PhoCl was cleaved and the RGD motif was released, resulting in detachment of the binding cells. The regulatory effect of the light illumination showed a time-dependent and cell density-dependent manner. green pigeon peas health benefitsWebbGrowth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name … green pig animal crossingWebb7 sep. 2024 · A topological variant of mMaple, named “PhoCl”, was reported by Zhang et al. in 2024 . To achieve the light-induced dissociation, PhoCl was engineered via circular permutation, followed with screening for variants with loss of … green pig 52c live tank treatmentWebbThe intact PhoCl-MBP fusion (75 kDa) was eluted first from the column at a volume of ∼47 mL. This was followed by the cleaved peptide-MBP fusion (45 kDa) at ∼52 mL and then … flystation viernheimDNA encoding the NES of MAPKK (NLVDLQKKLEELELDEQQ)26 and the NLS of SV40 (PKKKRKV)27were separately appended by PCR to the 5′ end of the gene encoding PhoCl. The gene encoding mCherry was inserted between the KpnI and XhoI sites of pcDNA3.1(+) (Thermo Fisher Scientific). Genes encoding NES … Visa mer Plasmid construction was performed through standard restriction-enzyme cloning. Pfu (Thermo Fisher Scientific), Taq (New England BioLabs), and Q5 (New England BioLabs) DNA polymerases were used for polymerase … Visa mer Overlap PCR was used to circularly permute the gene encoding mMaple, such that the original termini were joined with a GGSGG linker, and new … Visa mer All cell lines were grown from original frozen stocks without authentication or mycoplasma detection. The HEK cell line is listed in the International Cell Line Authentication … Visa mer The genes for mMaple, PhoCl, and PhoCl-MBP in pBAD/His B were used to transform E. coli strain DH10B or Top10. LB medium supplemented with 100 μg/mL ampicillin was inoculated with these transformants, and the … Visa mer green pig cleaning servicesWebbPhoCl is a new member of this class that works via a different mechanism - the PhoCl protein is irreversibly cleaved when exposed to light. Robert Campbell’s lab illustrates the … green pig country marketWebbPhotocleavable Cadherin Inhibits Cell-to-Cell Mechanotransduction by Light. ACS Chem Biol, 2024 Genetically Encoded Photocleavable Linkers for Patterned Protein Release … fly status jfk