Webb8 aug. 2024 · HiLyte647-labeled microtubule extensions were polymerized for 8 minutes at 30°C at 12 μM porcine brain tubulin (Cytoskeleton) containing 20% HiLyte647-labeled … WebbPE-Cy7-, HiLyte647- and/or APC-conjugated antibodies for 30 min. Biotin-conjugated antibodies were visualized with PE-Cy7- or APC-labeled streptavidin (SAv) …
FRET study in oligopeptide-linked donor-acceptor system in PVA …
WebbSynonyms: HiLyte™ Fluor 647 acid, NHS ester. HiLyte™ Fluor 647 acid, SE is an excellent fluorescent labeling dye that generates the protein conjugates that are slightly red … WebbIn this application note we show the performance of the PHERAstar FSX microplate reader from BMG LABTECH in Transcreener ® assays. In validation measurements the output … shinydocs logo
Controlled Endolysosomal Release of Agents by pH-responsive …
WebbWe selected TAMRA as the donor and HiLyte647 as the acceptor for our FRET pair (Figure 1). Both fluorophores are commercially available, well characterized, have a … Webb28 dec. 2024 · Time lapse movie of Rhodamine labeled NTA vesicles (yellow, 3 mol % Ni-NTA) above HiLyte647 microtubules (blue) in the presence of 500 nM KIF13B-6xHis. … Webbterminus; bottom) of taxol-stabilized HiLyte647-labeled microtubules incubated with 200 µg/ml subtilisin at 37 ° C for the indicated duration of time. Control sample was … shinydocs corporation