Hemolysin phospholipase a

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August undefined, 2024

WebPseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plastic when … Webdirect" (11) hemolysin phospholipase A (12). The importance of this experiment reached beyond the field of hymenop-tera venoms. As was shown later, some elapid (for example, cobra) venoms contain, besides phospholipase A, a "direct lytic factor" (see 13-15) which is probably identical with cardiotoxin (16). Two properties of the melittin mole-

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WebPolyvalent cations, in particular, ruthenium red, peptide HR-1, mellitin and cytotoxins Us-1 and Us-5 synergetically increase the hemolytic effect of phospholipase A2. During … Web1 feb. 1997 · A hemolysin was purified from a Vibrio cholerae O139 strain which moved as a single protein band of 67 kDa in SDS-PAGE. The hemolysin showed high level of … thomas old slow coach

Virulence Constitution of Multi-Drug-Resistant

Web13 mei 2024 · Furthermore, the effect of melittin on [Ca 2+]i could be mediated by a phospholipase A 2 (PLA 2) activation (Catisti et al. 2000). In support of this, it was shown that melittin-induced Ca 2+ influx in procyclic trypomastigotes of T. b. brucei was inhibited using 5 μM of 3-(4-octadecyl)-benzoylacrylic acid (OBAA), a PLA 2 inhibitor (Catisti et al. … WebPhobalysin P (PhlyP, for photobacterial lysin encoded on a plasmid) is a recently described small f-pore forming toxin of Photobacterium damselae subsp. damselae (Pdd). This organism, belonging to... WebThe hemolytic activity exhibited by pPHDD1-negative strains was caused by a chromosome-encoded hemolysin termed hlyA ch (PhlyC) and a phospholipase termed PlpV [ 13 ]. Recently, the immunogenicity of Hly ch was evaluated, revealing that the virulence might be further enhanced in PDD with the Hly ch gene [ 14 ]. uhw main switchboard

In vivo synergy of cardiotoxin and phospholipase A2 from the …

Hemolysin phospholipase a

Characterization of a haemolytic phospholipase A2 activity in …

Webphospholipase D is similar to the PLD of the brown recluse spider, which explains the presence of pain and edema at the site of infection. The synergistic activity of C. pseudotuberculosis exotoxins with the exotoxins of Rhodococcus equi in lysing red blood cells in agar forms the basis for the synergistic hemolysis inhibition (SHI) test. Web1 jun. 1994 · Phosphatidylinositol‐specific phospholipase C of Bacillus cereus: cloning sequencing and relationship to other phopholipases. Journal of Bacteriology ... Sphingomyelinase of Bacillus cereus as a bacterial hemolysin. Journal of Toxicology—Toxin Reviews ...

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Web3 mrt. 2024 · Bacteriol. 91:595-600. 1966.-The effect of streptolysin S, the group D hemolysin, and phospholipase C (the alpha toxin of Clostridium perfringens) on whole cells and spheroplasts or protoplasts of three strains of streptococci and Micrococcus lysodeikticus was tested. Viability, C (14)-glycine uptake, and lysis were measured. WebThe pathogenic process of PFCs normally involves forming channels or pores at the target cells' membranes. Note that the pores can have many structures. A porin -like structure …

WebRestriction mapping and Southern blot analysis revealed that lec, hlyA (hemolysin), and hlyC (lipase) are adjacent on the V. cholerae chromosome, and chromosomal digests of several El Tor, classical, and O139 (Bengal) strains demonstrated conservation of this gene arrangement [24]. WebVirulence factors Common toxins produced by C. perfringens: Alpha toxin: Lecithinase (or phospholipase) that breaks down cell membrane resulting in cell death and tissue necrosis. This toxin is also hemolytic and cardiotoxic. Beta toxin: Necrosis of tissue Delta toxin: Hemolysin Epsilon toxin: Acts to increase cell membrane permeability; permease. Iota …

WebModulation of the Activity of Secretory Phospholipase A 2 by Antimicrobial Peptides Hongxia Zhao and Paavo K. J. Kinnunen* Helsinki Biophysics & Biomembrane Group, Institute of Biomedicine, FIN-00014 University of Helsinki, Finland Received 3 September 2002/Returned for modiﬁcation 15 October 2002/Accepted 23 December 2002 WebSecretory phospholipase A2 (sPLA2) type IIA and X was analyzed in tumors from 22 patients with colon adenocarcinomas in order to determine the involvement and activity of sPLA2 in colon cancer. Evaluation of immunoreactive sPLA2 IIA by Western blotting showed a significantly higher level in the periphery of the tumors, compared to central tumor …

WebIn vitro experiments indicated that protease, phospholipase-A2, hemolytic activity and hemorrhage inducing activity of the venom were inhibited completely at a ratio of 1:20 (venom: QR) w/w. At the same concentration, the edema ratio was drastically reduced from 187% to 107%. Significant inhibition ...

WebPhospholipase A2 as a probe of phospholipid distribution in erythrocyte membranes. Factors influencing the apparent specificity of the reaction John K. Martin , Madan G. Luthra , Michael A. Wells , Richard P. Watts , and Donald J. Hanahan Cite this: Biochemistry 1975, 14, 25, 5400–5408 Publication Date: December 16, 1975 thomas oliemans concertgebouwWeb16 dec. 2009 · The cytotoxic and hemolytic activities of PhlA both required phospholipids as substrates. Conclusion We have shown that the S. marcescens phlA gene produces hemolysis on human blood agar plates. PhlA induces destabilization of target cell membranes in the presence of phospholipids. uhw liveWeb26 jan. 2024 · Background: Medusozoans utilize explosively discharging penetrant nematocysts to inject venom into prey. These venoms are composed of highly complex proteins and peptides with extensive bioactivities, as observed in vitro. Diverse enzymatic toxins have been putatively identified in the venom of jellyfish, Nemopilema nomurai and … uhw medical physicsWebPseudomonas aeruginosa produces at least two distinct hemolysins: heat-labile hemolysin, which is known as phospholipase C, and heat-stable hemolysin, which is considered to … thomas oliemans formidableWebThe mode of action of highly punned beta hemolysin derived from the R-1 strain of Staphylococcus aureus has been investigated. Sphingomyelin was absent from lipid extracts of sheep erythrocytes treated with beta hemolysin when compared to normal cells. thomas oliemans wikipediaWebPhospholipase A2 from porcine pancreas (Lecithinase A ); ammonium sulfate suspension, main >= 600units/mg protein; Phospholipase A2 has been used in phospholipase assay and to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation; Phosp uhw medicines information uhw medical records